MECHANISMS OF HELPER PHAGE. DEPENDENT TRANSFECTION IN BACILLUS SUBTILIS

Abstract

Bacteriophage SP-18 rendered competent cells of Bacillus subtilis 168 (ind(-)) susceptible to transfection by DNA from apparently unrelated phages. DNA from SP-18 was also active as helper, but it was less effective than the intact phage. Sheared SP-18 DNA retained its helper effect. Phage SP-18 inactivated by ultraviolet irradiation helped SP-10 DNA, but not LP-19 DNA, transfect competent 168 cells.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1968
Accession Number
AD0828766

Entities

People

  • Darrel D. Gwinn
  • William D. Lawton

Tags

DTIC Thesaurus Topics

  • Acids
  • Bacteria
  • Bacteriophages
  • Biological Sciences
  • Biomedical And Dental Materials
  • Deoxyribonucleic Acids
  • Films
  • Infection
  • Macromolecules
  • Microorganisms
  • Molecular Weight
  • Molecules
  • Nucleic Acids
  • Polymeric Films
  • Polymers
  • Transfection
  • Wound Infections

Fields of Study

  • Biology
  • Chemistry

Readers

  • Immunology
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Molecular Genetics