A STUDY OF 'IMMEDIATE' SENSITIZATION BY ADSORPTION OF ANTIGENS AND ANTIBODIES IN VITRO

Abstract

The interaction of several sea urchin toxin preparations with certain substrates in mammalian serum produces dialyzable, heat-stable substances which cause contractions of the guinea pig's ileum and rat's uterus. Evidence is presented to show that the toxin acts kinetically as an enzyme and that one of the substrates is electrophoretically pure human alpha 2-macroglobulin. Crude toxin preparations were also shown to be kininolytic with respect to the dialyzable reaction-product as well as to synthetic bradykinin. Rabbit antibodies to formalinized toxoids quantitatively precipitated active toxin, fixed complement, and protected mice against lethal intravenous doses in the usual quantitative protection and neutralization tests. Immunoelectrophoretic analysis brought out the existence of two distinct antigenic determinants, and additional serological tests showed that antibodies directed against the pedicellarial toxins of T. gratilla cross-reacted with test proteins of S. purpuratus.

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Document Details

Document Type
Technical Report
Publication Date
Feb 26, 1968
Accession Number
AD0829724

Entities

People

  • George A. Feigen

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Antibodies
  • Biological Factors
  • Biomedical And Dental Materials
  • Blood
  • Blood Proteins
  • Chemical Synthesis
  • Chemistry
  • Immune Serums
  • Polymer Chemistry
  • Polymeric Films
  • Proteins
  • Proteomics
  • Rodents
  • Sea Urchins
  • Toxicity

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Molecular and Cellular Biochemistry