FRACTIONATION OF EASTERN EQUINE ENCEPHALITIS VIRUS BY DENSITY GRADIENT CENTRIFUGATION IN CSCL

Abstract

Experiments in which partially purified eastern equine encephalitis (EEE) virus was centrifuged to equilibrium in CsCl revealed three bands. These consisted of a hemagglutinating particle, rho = 1.18; a major infectious band, rho = 1.20; and a minor infectious band, rho = 1.23. Analysis of radioactive profiles of CsCl-fractionated EEE, labeled with either P32O4 or uridine-H3, indicated that most of the hemagglutinin was stripped from the complete virion. The viral origin of the hemagglutinin was verified by inhibition with specific antiserum. Attempts to differentiate EEE rho = 1.23 from the complete virion (rho = 1.20) showed that the heavier particle was neither a viral contaminant, a density mutant, nor an immature form of the virus. Results from a CsCl rebanding experiment support the fact that EEE rho = 1.23 was produced by a CsCl-induced alteration or degradation of the complete virion.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1968
Accession Number
AD0832598

Entities

People

  • Arthur Brown
  • Edwin J. Hoffman
  • Halvor G. Aaslestad

Tags

Communities of Interest

  • C4I
  • Counter IED

DTIC Thesaurus Topics

  • Albumins
  • Arboviruses
  • Biological Sciences
  • Cells
  • Chemical Analysis
  • Chemistry
  • Degradation
  • Encephalitis
  • Environmental Pollutants
  • Equine Encephalitis
  • Fractionation
  • Immune Serums
  • Inhibition
  • Materials
  • Virion
  • Virology
  • Viruses

Fields of Study

  • Biology

Readers

  • Materials Science and Engineering.
  • Virology (or Medical Virology).