INHIBITION OF STAPHYLOCOCCAL ENTEROTOXIN B FORMATION BY CELL WALL BLOCKING AGENTS AND OTHER COMPOUNDS

Abstract

Enterotoxin B formation by Staphylococcus aureus S6 was inhibited by Tween 80, oleic acid, sodium deoxycholate, penicillin, D-cycloserine, or bacitracin. Toxin formation by strain 243 was sensitive to oleic acid, sodium deoxycholate, sodium lauryl sulfate, D-cycloserine, or bacitracin. The effect of D-cycloserine was reversed by D-alanine with strain 243 but not with strain S6. Neither penicillin nor bacitracin inhibited alpha-hemolysin or coagulase activity of strain S6; however, 0.118 micromoles of D-cycloserine per ml of liquid medium increased the alpha-hemolysin titer more than eightfold. Pigmentation of strain 243 was reduced by oleic acid, sodium deoxycholate, or methicillin and was completely inhibited by D-cycloserine or bacitracin. Glucose was required for the inhibition by spermine of valine-C14 incorporation into cellular protein of strain S6. It is suggested that these data indicate that the cell surface may contain sites important to the synthesis of enterotoxin B.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1968
Accession Number
AD0833372

Entities

People

  • Mischa E. Friedman

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Alanine
  • Anti-Bacterial Agents
  • Bacteria
  • Biological Sciences
  • Cells
  • Cellular Structures
  • Chemistry
  • Culture Media
  • Fungi
  • Inhibition
  • Inhibitors
  • Materials
  • Oleic Acid
  • Proteins
  • Staphylococcus Aureus

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology
  • Molecular and Cellular Biochemistry