IMMUNOFLUORESCENT METHODS IN THE DIAGNOSIS OF INFECTIOUS DISEASES

Abstract

The fluorescent antibody methods are utilized in microbiological diagnosis to determine either (a) the presence of unknown antigens (usually microorganisms) in tissue sections or smears, or (b) the presence of an unknown antibody in the serum of the patient. There are four methods by which one or both objectives can be achieved: (1) Direct; (2) Indirect; (3) Inhibition, and (4) Complement staining. The direct and indirect methods have been used to a greater extent than the last two procedures and are discussed with more detail. Since the principles of IF depend on the method used, they are also described in the report.

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Document Details

Document Type
Technical Report
Publication Date
Sep 27, 1966
Accession Number
AD0834335

Entities

People

  • Charles M. Carpenter
  • Frank Fazzan
  • James N. Miller
  • Ruth A. Boak

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Antigen Antibody Reactions
  • Antigen-Antibody Complex
  • Antigens
  • Blood
  • Detection
  • Diarrhea
  • Dyes
  • Fluorescence
  • Immune Serums
  • Infectious Diseases
  • Laser Dyes
  • Materials
  • Microorganisms
  • Public Health
  • Rodents
  • Veins

Fields of Study

  • Biology
  • Medicine

Readers

  • Microbial Pathology
  • Systems Analysis and Design