IMMUNOFLUORESCENT IDENTIFICATION OF CL PERFRINGENS IN VARIOUS SECRETIONS
Abstract
A study was conducted to determine whether Cl. perfringens may be demonstrated in various human secretions (pus, sore secretion, punctate, etc.) by the immunofluorescent method, using no preliminary preparation, because the usual anaerobic culturing method is very lengthy and may require several days or weeks. 100 different secretions were tested, and for the purpose of comparison the usual bacteriological culture was also performed by the anaerobic method. As a result of the tests, four percent positive indications were obtained with the anaerobic culture method, compared to 19 percent positive results with the immunofluorescent method. Thus the advantage of the immunofluorescent method in demonstration of Cl. perfringens in various secretions consists not only of reducing the test time from several days or weeks to two or three hours, but also of the much greater sensitivity of the method.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1966
- Accession Number
- AD0834623
Entities
People
- Peter Geck
- Rozsa Szanto
Organizations
- United States Army Biological Warfare Laboratories