INFLUENCE OF CENTRIFUGATION ON AGGLUTINATION OF SALMONELLA,
Abstract
The findings of a number of earlier authors on the influence of centrifugation on the agglutination of microbes were confirmed and extended with observations on the influence of temperature, time and centrifugal force. It was shown that the H-agglutination of salmonella was accelerated slightly but not essentially. By contrast, O-agglutination of salmonella, Shigella, coli and brucella is basically accelerated. In practice, it is possible, sometimes at a temperature of 37C but always at 45C, to obtain the full titer of the serum after incubation for 2 hours of the antibody with the antigen whereas the same result without centrifugation requires 24 hours. With hyperimmune sera, it was not possible to obtain a higher titer with centrifugation than without centrifugation. By contrast, in sera of patients showing a negative reaction to the customary agglutination test, a positive result was obtained with the salmonella antigens 0 9,12 and 4,5 and sometimes also in titers 1 : 40 and higher for 5 to 16% of the sera. Although the temperature of 50C is somewhat more favorable in the method without centrifugation, it produces the same and perhaps even less satisfactory results with centrifugation. The quality of agglutination is a function of the number of revolutions. Most favorable were 2,000 rpm during an interval of 3 to 5 minutes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 09, 1965
- Accession Number
- AD0835140
Entities
People
- Mandlikova Zdenka
- Vladimir Wagner
Organizations
- United States Army Biological Warfare Laboratories