STUDY OF THE VARIABILITY OF THE TICK-BORNE VIRUS OF ENCEPHALITIS. REPORT 1. LONG CULTIVATION OF VIRUSES OF THE TICK-BORNE ENCEPHALITIS GROUP IN RENAL- CELL CULTURE OF PIG EMBRYO AND IN CHICK EMBRYOS

Abstract

As a result of the prolonged passaging in a culture of pig-embryo renal cells at 33C (70 passages) and at 40C (80 passages), as well as in developing chick embroys (120 passages) of the viruses of the tick-borne encephalitis group (strains Ix-10, Pan, and I-40), the neuropathogenic properties of these strains for white mice have not been changed. As a result of the adaptation, certain strains have acquired the aptitude to destroy SOTs cells and fibroblasts of chick embryos. Pan and I-40 strains, both the initial and the variants obtained, have possessed the property to form plaques in culture of pig-embryo renal cells. Variants of the viruses, obtained at growing in cultures of pig-embryo renal cells at 40C (Pan and I-40), at 33C, and in thick embryos (I-40), have acquired the capacity to form plaques in cultures of SOTs cells.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1965
Accession Number
AD0835146

Entities

People

  • L. G. Stepanova
  • N. N. Bogomolova
  • O. G. Andzhaparidze
  • R. G. Deciatskova

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cells
  • Commerce
  • Culture Techniques
  • Diameters
  • Dilution
  • Encephalitis
  • Export Controls
  • Exports
  • Fibroblasts
  • Inoculation
  • International Trade
  • Literature
  • Maryland
  • Materials
  • Translations
  • Viruses

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).