CULTIVATION OF LISTERIA MONOCYTOGENES
Abstract
Listeria monocytogenes was demonstrated in soil, milk and serum tested after 1 3/4 years, in sand tested after 10 months, and in tap water tested after 1 year. Growth was also obtained from nutrient media months after they had been inoculated with pure cultures. This was not altered by secondary staphylococcal or sporogenic infection of the material. Cold-culture was of limited value in getting a take of Listeriae in coliform-overgrown nutrient media, and of no value in milk, soil, and sand cultures. Keeping the milk at room temperature led to a reduction of the coliform organisms after some weeks, and to pure cultures of Listeriae. Enrichment of cultures by preservation at room temperature or by keeping them in the cooling cupboard is very time consuming. Rapid and satisfactory selective culture is obtained with the help of anti-gram negative sera. Both coli and proteus colonies were inhibited by this means, so that Listeriae were isolated without difficulty. Ps. pyocyanea showed more resistance to bactericidal serum; it was, however, possible to isolate the Listeriae from the majority of mixed cultures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 30, 1966
- Accession Number
- AD0835164
Entities
People
- Bernd Warnecke
Organizations
- United States Army Biological Warfare Laboratories