SPECIFIC IDENTIFICATION OF FRACTION-I-POSITIVE PASTEURELLA PESTIS COLONIES ON ANTISERUM-AGAR PLATES

Abstract

A method is described for using antiplague serum in blood agar base plating media to detect Fraction-I-positive Pasteurella pestis. The antiserum was produced conveniently and in large volume in rabbits utilizing Cutter plague vaccine combined with Freund's complete adjuvant. P. pestis colonies were specifically identified within 48 hours after plating by the presence of a precipitin ring surrounding each colony. The basis of the test was shown to be a precipitin reaction between Fraction I antigen released from P. pestis colonies after chloroform vapor treatment and Fraction I antibody present in the antiserum-agar medium.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1968
Accession Number
AD0835494

Entities

People

  • Johnnie M. Albizo
  • Michael J. Surgalla

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Agglutination
  • Animals
  • Antibodies
  • Antigen Antibody Reactions
  • Antigens
  • Biological Sciences
  • Cells
  • Chemistry
  • Chloroform
  • Identification
  • Immune Serums
  • Laboratory Animals
  • Materials
  • Production
  • Standards
  • United States
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology

Technology Areas

  • Biotechnology