EXTRACTION OF BACTERIA WITH PHENOL/WATER,

Abstract

Two simple procedures are reported for the extraction of bacteria with phenol/water. After gram-negative bacteria are treated with phenol/water emulsions for a few minutes, chilled, the somatic glycoproteides (principally O antigens) are obtained in the watery phase in practically 100% yield. After extraction of the bacteria with warmed, homogeneous phenol/water mixtures and separation of the phases under refrigeration the protein-free polysaccharides are found together with the nucleic acids in the watery phase. Some chemical and immunological characteristics of the glycoproteides and polysaccharides obtained by the two procedures are described. (Author)

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1963
Accession Number
AD0836109

Entities

People

  • Fritz Bister
  • Otto Luederitz
  • Otto Westphal

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Acids
  • Bacteria
  • Biomolecules
  • Chemical Compounds
  • Emulsions
  • Extraction
  • Germany
  • Gram-Negative Bacteria
  • Lipopolysaccharides
  • Nucleic Acids
  • Polysaccharides
  • West Germany

Readers

  • Analytical Chemistry
  • Immunology
  • Speech Processing/Speech Recognition.

Technology Areas

  • Biotechnology