QUANTITATIVE MICROANALYSIS OF AMINO ACIDS IN PROTEINS,
Abstract
The importance of micromethods in the identification and evaluation of proteins is discussed. Nitrogen content of protein hydrolysates was determined faster and more accurately than heretofore by using an alkaline permanganate solution as an oxidizer. A simple, systematic method for determining the amino acids known to be essential to life was worked out. The process consists in separating the basic amino acids using phosphotungstic acid, determining the hexone bases by the Van Slyke method, separation of the aliphatic from the aromatic amino acids using active charcoal, and colorimetric determination of the amino acids. The total time required for the analysis when using barium hydroxide to break down the proteins is 13 hours. The quantity needed to determine all the amino acids is about 200 to 300 mg. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0836117
Entities
People
- G. Gorbach
Organizations
- United States Army Biological Warfare Laboratories