FLUORESCENT-SEROLOGICAL DIAGNOSIS OF BOVINE LEUKOSIS
Abstract
The principles and methods for marking antibodies with fluorescent dyes are described. The bovine leukosis virus was adapted to mice and is under experimental control. By means of the 'indirect method' leukosis-specific particles were found in smear samples of mouse tumors and in blood samples of cattle with tumorous or hematological leukosis, and also in blood samples of cattle which did not yet show any hematological changes. The same method in a similar application identified leukosis-specific antibodies in the serum of cattle. The statistical evaluation of the results of the investigation of altogether 510 cows from 29 leukotic herds gave a highly significant Wilcoxen test for the distribution of the fluorescent-serological antigen and antibody identification with regard to the absolute number of leucocytes in all cows over three years old. In the same way, the correlation between the fluorescent-serological result and the hematological judgment according to the leukosis key was found to be highly significant.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1963
- Accession Number
- AD0836217
Entities
People
- A. Tolle