BIOCHEMICAL STUDIES ON RICE BLAST DISEASE. PART 10. THE BIOCHEMICAL CLASSIFICATION OF PIRICULARIA ORYZAE CAVARA (6)
Abstract
Forty-seven strains of piricularia oryzae were cultivated in a thiamine-free synthetic culture medium, and in a synthetic medium to which nicotinic acid was added. Nine strains reproduced rather well in a thiamine-free synthetic medium, and these strains produce 0.17-2.91 grams/ml of nicotinic acid in the culture filtrates. The other strains require thiamine. It was shown that there is excellent growth of piricularia oryzae in general in a complete synthetic medium to which thiamine has been added. Also, eight strains developed relatively well in a medium to which nicotinic acid was added in place of thiamine, and it was established that nicotinic acid can be employed here as a substitute for thiamine. Strains were cultivated which require thiamine in a complete culture medium to which thiamine was added, and found that from 0.11 to 2.64 grams/ml of nicotinic acid was produced. On the basis of the results the system of biochemical classification of piricularia oryzae was revised into 13 types.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 11, 1964
- Accession Number
- AD0836236
Entities
People
- Hiroshi Otsuka
- Kinjiro Tamari
- Nagahiro Ogasawara
Organizations
- United States Army Biological Warfare Laboratories