USE OF SEPHADEX FOR RAPID PURIFICATION OF FLUORESCENCE STAINED ANTIBODY SOLUTIONS
Abstract
Quantitative separation of the unbound fluorescence dye from antibody solutions labelled with fluorescence is achieved by the use of gel filtration with Sephadex G 50. As compared with the previously used dialysis, the purification is terminated in a few minutes and therefore can be done at room temperature. Due to the good possibility of optical control, it permits a regain without loss of the serum conjugate with only negligible decrease of concentration.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1963
- Accession Number
- AD0836697
Entities
People
- Michael J. Wagner
Organizations
- United States Army Biological Warfare Laboratories