NEW POSSIBLE METHODS OF MAKING FLUORESCENCE-MARKED PROTEINS

Abstract

Besides the usual sulfochlorides, several fluorescent dyes containing sulfo groups were bound to proteins. The most favorable conditions for this were determined, and the marked proteins were checked for purity by electrophoresis. The fluorescence spectrum of the dyes and a few conjugates were measured. By means of protein markings of different colors the possibilities for diagnostical, immunological and histochemical applications were extended and improved. The method described is a rather simple way of making fluorescin marked proteins.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1963
Accession Number
AD0837212

Entities

People

  • H. Uehleke

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Antibodies
  • Azo Compounds
  • Dyes
  • Emission
  • Emission Spectra
  • Fluorescence
  • Fluorescent Dyes
  • Isocyanates
  • Low Temperature
  • Microbiology
  • Microorganisms
  • Mixtures
  • Organic Solvents
  • Proteins
  • Spectra
  • United States
  • West Germany

Fields of Study

  • Chemistry

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular and Cellular Biochemistry
  • Theoretical Analysis.