CONFIGURATION AND LONGITUDINAL DISTRIBUTION OF DNA MOLECULES IN SOLUTION,

Abstract

A diffusion method is described for visualization of DNA molecules which is equivalent to the spreading of a mixed DNA-protein solution. Macromolecules diffusing in a solution of about 5 x 10 to the minus 8th power g DNA per ml are adsorbed by a cytochrome c-film at the air-water interface. Subsequently they are transferred to electron microscopic supports. In the enlarged two-dimensional image the end-to-end distances and the length distribution are measured for different DNA preparations (from cod fish, trout, holothuria and T2 phage). The molecular shape is not a random coil or a rod but intermediate on account of electrostatic interaction. (Author)

Document Details

Document Type
Technical Report
Publication Date
Oct 18, 1965
Accession Number
AD0837468

Entities

People

  • Albrecht K. Kleinschmidt
  • Dimitrij Lang
  • Rudolf K. Zahn

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Cytochromes
  • Diffusion
  • Electrons
  • Macromolecules
  • Molecules
  • Netherlands
  • Two Dimensional
  • Visualizations

Readers

  • Combustion and Flow Dynamics.
  • Immunology
  • Molecular and Cellular Biochemistry

Technology Areas

  • Microelectronics