STUDIES OF TOBACCO MOSAIC VIRUS WITH FLUORESCENT ANTIBODIES
Abstract
A method for the study of plant tissue with the aid of fluorescent antibodies is described. Tobacco plants are examined at known intervals after infection with tobacco mosaic virus. Viral protein is demonstrable already 45 hours after infection. It appears first in the cytoplasm, in a perinuclear zone, and spreads subsequently over the entire cytoplasm. No virus protein is seen in the nuclei and chloroplasts. Synthesis of viral protein consequently seems to occur exclusively in the cytoplasm. This fact is supported also by fractionation of infected tissue. Here the fluorescent antibody reveals viral antigen principally in the microsomal fraction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0838281
Entities
People
- B. Roettger
- G. Schramm
Organizations
- United States Army Biological Warfare Laboratories