STUDIES ON ARBORVIRUSES AND THEIR EXTRACTS BY MEANS OF CHROMATOGRAPHY ON ION-EXCHANGE CELLULOSES AND GEL-FILTRATION ON AGAROSE

Abstract

Chromatography on ion exchange cellulose permits the separation of the virus studied. No soluble hemagglutinin has been found. Gel-filtration on agarose columns demonstrates a homogeneous behavior of infectious and hemagglutinating properties of the original preparations. Viral particles break when subjected to the action of ether and detergents; the break is associated with loss of infectious capacity only; hemagglutinating units obtained with this method preserve certain characteristics of the virions from which they are issued (immunologic specificity, inactivation, characters). The significance of these findings for the virion architecture is discussed.

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Document Details

Document Type
Technical Report
Publication Date
Nov 29, 1965
Accession Number
AD0838554

Entities

People

  • J. Nicoli

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Blood
  • Cells
  • Cellulose
  • Culture Media
  • Encephalitis
  • Ethers
  • Filtration
  • Immune Serums
  • Inhibitors
  • Ion Exchange
  • Materials
  • Rodents
  • Tissue Culture
  • United States
  • Virion
  • Viruses
  • Yield

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design
  • Virology (or Medical Virology).