A METHOD FOR PREPARING SMALL QUANTITIES OF C14-LABELED STAPHYLOCOCCAL ENTEROTOXIN B

Abstract

A procedure is described for production from Staphylococcus aureus of small amounts of radioisotopically labeled staphylococcal enterotoxin B for animal metabolism studies. The procedure is a modified scaled-down adaptation of the Schantz method of enterotoxin production. The organism is cultured on medium containing C 14-labeled yeast protein hydrolyzate, and the toxin is purified by adsorption on and elution from Amberlite CG-50 resin columns. The procedure is capable of yielding 12 to 16 mg of C 14-labeled enterotoxin B of high purity (96 to 100%) with an isotope concentration of 0.2 microcurie per mg.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1968
Accession Number
AD0838752

Entities

People

  • John P. Bowden

Tags

DTIC Thesaurus Topics

  • Adsorption
  • Anatomy
  • Animals
  • Antibodies
  • Biochemistry
  • Biological Sciences
  • Chemistry
  • Immune Serums
  • Isotopes
  • Laboratory Animals
  • Materials
  • Metabolism
  • Production
  • Proteins
  • Staphylococcus Aureus
  • Uss Valley Forge
  • Veins

Readers

  • Analytical Chemistry
  • Immunology
  • Military/Explosive Ordnance Disposal (EOD) Technology