AN AUTOMATED HEMAGGLUTINATION TECHNIQUE FOR ESTIMATING THE ACTIVITY OF TYPE A BOTULINUM TOXIN

Abstract

A quantitative relationship between hemagglutination titer and animal toxicity of type A botulinum toxin was demonstrated utilizing a modification of the hemagglutination procedure of Rosenfield et al. Hemagglutination titer was correlated to animal toxicity by measuring hemagglutinin activity of known concentrations of toxic material with an established mouse intraperitoneal LD50 value. The automated procedure employed is accurate and reproducible in the range of 5 to 50 micrograms toxin per ml.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1968
Accession Number
AD0838771

Entities

People

  • James B. Williams

Tags

DTIC Thesaurus Topics

  • Agglutination
  • Analysis Of Variance
  • Animals
  • Antibodies
  • Antitoxins
  • Cells
  • Confidence Limits
  • Control Systems
  • Inhibition
  • Laboratory Animals
  • Maryland
  • Materials
  • New York
  • Public Health
  • Rodents
  • Toxicity
  • United States

Fields of Study

  • Biology

Readers

  • Immunology
  • Regression Analysis.