INFECTION OF BACILLUS SUBTILIS 168 WITH BACTERIOPHAGE SP-10 AFTER INDUCTION BY MITOMYCIN C OR ULTRAVIOLET LIGHT,
Abstract
Strains of Bacillus that harbor defective phage PBSX were insensitive to SP-10(V), although the phage adsorbed to these insensitive strains. Strains that did not carry the defective phage were sensitive to SP-10(V). B. subtilis 168 ind, which can be transduced by SP-10(V) but is nonpermissive for this phage, was rendered phage-sensitive after treatment with ultraviolet (UV) light or mitomycin C. Following induction with UV light, maximal sensitivity to SP-10(V) was obtained at a multiplicity of infection (MOI) of approximately 14; with mitomycin C induction, an MOI of approximately 1.0 was required. Phage SP-19(V) maturation in sensitized cells was followed by plating infected streptomycin-sensitive cells in the presence of streptomycin at various stages during phage development. The latent period was estimated at 60 to 75 minutes. We suggest that the resistance of B. subtilis 168 to SP-10 is controlled, at least in part, by the presence of a defective prophage. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1968
- Accession Number
- AD0838814
Entities
People
- Ivan D. Goldberg
- Theodore Bryan