SMALL PLAQUE MUTANTS OF THE SINDBIS VIRUS

Abstract

In embryo cell cultures in gel medium infected with the Sindbis virus, two types of plaques appear: large (G) and small (p) plaques. The G plaques appear in 24 hours and grow until they attain a diameter of more than 20mm. The small plaques (p) appear after 48 hours but never attain a diameter exceeding 3mm. After three successive reinoculations stable sub-lines of the p type are obtained, while the G sub-lines always give rise to a predominance of G but a small, constant percentage of p plaques. The interpretation of this phenomenon is that a mutation is spontaneously occurring, giving rise to small- plaque mutants from clones of wild-type, large-plaque viruses.

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Document Details

Document Type
Technical Report
Publication Date
Nov 05, 1965
Accession Number
AD0838829

Entities

People

  • Cl. Hannoun
  • J. Asso
  • P. Ardoin

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Cells
  • Culture Media
  • Culture Techniques
  • Demographic Cohorts
  • Diameters
  • Export Controls
  • Growth Factors
  • Incubation
  • Inoculation
  • Mutations
  • Tissue Culture
  • Tissue Culture Cells
  • United States
  • Virion
  • Virology
  • Viruses

Fields of Study

  • Biology

Readers

  • Combustion Dynamics and Shock Wave Physics.
  • Theoretical Analysis.
  • Virology (or Medical Virology).