STUDIES ON THE POLYSACCHARIDE ANTIGENS OF BRUCELLA

Abstract

In this report a polysaccharide fraction isolated from a smooth Brucella abortus B99 strain by the formamide technique (Fuller's technique) was analysed. Its properties and chemical constitution have been compared with those of the polysaccharide fraction of the tubercle bacilli isolated under the same conditions and studied earlier (Fuks, 1958) because of the occurrence of cross reactions between them. The density, viscosimetry, optical rotation are different in both polysaccharides. Brucella polysaccharide contains uronic acid. The polysaccharide fraction has a behavior of a complex hapten, it does not contain protein, lipids or inositol. It does not dialyse and showed homogeneity when submitted to analytical ultracentrifuge, paper electrophoresis and immunolectrophoresis techniques.

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Document Details

Document Type
Technical Report
Publication Date
May 05, 1965
Accession Number
AD0838943

Entities

People

  • C. A. Serpa
  • M. A. Fuks

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Acids
  • Amines
  • Amino Acids
  • Carbohydrates
  • Chemical Analysis
  • Chemistry
  • Chromatographic Analysis
  • Glycogen
  • Health Services
  • Materials
  • Microbiology
  • Nucleic Acids
  • Polysaccharides
  • Redox Indicators
  • Sugar Alcohols
  • Sugars
  • Uronic Acids

Readers

  • Infectious Disease/Epidemiology
  • Molecular and Cellular Biochemistry
  • Regression Analysis.