THE LUMINESCENCE OF LUMINOL XI

Abstract

The luminol reaction may serve very well for the detection of blood spots and traces of blood on various objects in judicial-medical and criminological practice. There are various recipes for preparing the luminol reagent, among which that with sodium hydroxide yields equally intensive chemiluminescence regardless of whether it is a matter of fresh traces of blood (oxyhemoglobin) or dried spots (methemoglobin). The reagents with sodium carbonate or with sodium perborate, on the contrary, yield stronger luminescence with dried blood spots than with solutions of fresh blood under otherwise like experimental conditions. The clarity of this reaction is exceptionally great. The correct manner of effecting this reaction to detect blood spots is shown, and the results of the quantitative measurements concerning the influence of various natural substances on the chemiluminescence of luminol are given. Both catalytic and inhibitory effects were noted in the action of these substances.

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Document Details

Document Type
Technical Report
Publication Date
Aug 12, 1964
Accession Number
AD0839541

Entities

People

  • K. Weber
  • V. Mikulovic

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Aqueous Solutions
  • Carbonates
  • Catalysts
  • Chemiluminescence
  • Detection
  • Hemoglobin
  • Hydrogen
  • Hydroxides
  • Luminescence
  • Measurement
  • Reaction Time
  • Rocket Oxidizers
  • Sodium
  • Sodium Hydroxide
  • Vegetables
  • Vitamin C
  • Water

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Chemistry (specifically Chemical Fluorescence)
  • Systems Analysis and Design