NITROGENOUS COMPONENTS OF NUTRIENT MEDIUM FOR CULTIVATION OF CLOSTRIDIUM PERFRINGENS TYPE D
Abstract
The report concerns the dynamics of the nitrogenous components of the medium in the growth and toxin-formation of Clostridium perfringens of different toxicity. The following conclusions were drawn: (1) toxin formation in Cl- perfringens type D is related to the multiplication of the culture; (2) in culturing of toxigenic and weak-toxigenic strains of Cl-perfringens type D on a semi-synthetic medium based on a hybrid hydrolysate of casein, the level of total end protein nitrogen of the culture filtrates diminished, while the residual and amino-nitrogen increased in comparison with a sterile medium; (3) in the growth of a toxigenic strain there is a sharp increase in the quality of amino nitrogen in the medium during the period of intensive growth and toxin formation. In the filtrates of a culture of a weak-toxigenic strain, the level of nitrogen during the whole period of culturing was relatively stable. This regularity is related to the increased proteolytic activity of the toxigenic strain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0839633
Entities
People
- E. Sh. Zhuravel
Organizations
- United States Army Foreign Science and Technology Center