ON THE CLINICAL APPLICATION OF A NEW ELECTROPHORESIS PROCESS IN LIVER DISEASES

Abstract

In increasing measure interest in medical-biological research has recently focused on the proteins of human serum and plasma. While the constituents of blood have been intensively studied for some time the difficulties of methodology have slowed the trend to use the analysis of proteins and peptides in clinical cases. There is obviously a need for a simple, reproducible instrument suitable for a larger series of experiments. The instrument should also be inexpensive. Such an instrument recently developed showed that amino acids and proteins can be separated on filter paper treated with a buffer solution. The proteins and amino acids may be made visible by staining. In carrying this principle further, this investigation focused on developing a dye which would stain proportionally to the concentration of the protein on paper. This together with a special method to quantitate the process of the electrophorotic separation is now described and shows great promise as a commonly useful, routine method to be used in hospitals and clinics.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1968
Accession Number
AD0840033

Entities

People

  • M. Knedel

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Acids
  • Amino Acids
  • Blood Proteins
  • Buffers (Chemistry)
  • Cirrhosis
  • Diseases And Disorders
  • Electrophoresis
  • Filter Paper
  • Gamma Globulin
  • Globulins
  • Government (Foreign)
  • Health Services
  • Liver Diseases
  • Paper
  • Proteins
  • United States

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