CULTURING OF R. TSUTSUGAMUSHI IN TISSUE CULTURES,
Abstract
The culturing of the causative agents of rickettsial disease in tissue cultures was broadly used for the study of the morphology, growth processes, and multiplication of rickettsiae, for the study of the interrelations of rickettsiae with a cell, and for the accumulation of a great number of agents for the purpose of preparing an antigen. The following conclusions were drawn: (1) R. tsutsugamushi (strains B15, Gilliam, and B58) multiplied well in interbred L-cells and initially trypsinized fibroblasts of chicken embryo. Rickettsiae appeared on the third day, reaching its maximum around 7 - 9th day; (2) There was success in supporting a culture of R. tsutsugamushi by means of subinoubations in an interbred culture of L-cells (up to five incubations); (3) R. tsutsugamushi (strain B15) maintains its pathogenic properties for mice after four incubations on a cell culture; (4) Infection of a tissue was a sensitive test for the detection of R. tsutsugamushi in materials stored in dehydrated or frozen state. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0840370
Entities
People
- L. V. Tarasevich
- R. G. Dyvysaltseva
Organizations
- United States Army Foreign Science and Technology Center