IDENTIFICATION AND PURIFICATION OF A PEPTIDE SEGMENT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI BY COMPLEMENTATION IN VITRO
Abstract
The observations made in this report indicate that gene z contained at least two distinctive segments which determined the structure of different peptides whose association constituted the protomere of beta-galactosidase. Complementation studies in vivo have shown, among other things, that deletions with a feeble extension (M15 and 05) are weakly complementable with point mutants located between the markers G and 242. These observations suggested that the segment located between the operator and peptide 'omega' itself determined the structure of at least two distinctive peptides.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 12, 1967
- Accession Number
- AD0840846
Entities
People
- Agnes Ullmann
- David Perrin
- Francois Jacob
- Jacques Monod
Organizations
- United States Army Biological Warfare Laboratories