PURE EPITHELIAL CULTURES ON PLASTIC MEMBRANES WITHOUT TRANSPLANTING AND WITHOUT PLASMA: RAPID PREPARATION OF COLORABLE CULTURES AS SMEARS
Abstract
Humid plastic membranes form a particularly favorable surface for the rapid development of the epithelial membranes. Pure epithelial cultures may thus be obtained without transplanting and without plasma within three days. These cultures, devoid of explant, may be maintained in a vigorous state of proliferation by nutritive bathing. The technique allows the study of various actions (virus, Physicochemical factors) on epithelial preformed membranes, with a minimum of work. The obtained preparations, being devoid of plasma, may be stained and observed under immersion as smears. Certain applications are considered.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0841597
Entities
People
- Georges Barski
- John Wirth
Organizations
- United States Army Biological Warfare Laboratories