Pilot Experiment on the Detection of the Pathogenic Agents of the Fiessinger-Leroy Reiter Syndrome
Abstract
The chicken embryo is inoculated with vaccine, which is injected into the vitelline sac on the seventh day or into the chorioallantoic membrane on the eleventh day. The smears, which were exposed to vaccinated rabbit serum, made it possible to demonstrate fluorescent particles corresponding to viral antigens. The same smears did not exhibit any fluorescence when they were put in contact with normal sera. The smears, which were prepared from urethral secretion of patients with viral urethritis or from vitelline sac or chorioallantoic membranes of embryonic eggs inoculated with urethroprostatic secretions from these patients (positive culture) on the seventh day, were processed according to the technique of immunofluorescence and Giemsa and acridine-orange fluorescence. Serum from a patient with a urethro-conjunctivo-synovial syndrome of viral origin was utilized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 26, 1968
- Accession Number
- AD0842904
Entities
People
- Paul Galistin
Organizations
- United States Army Biological Warfare Laboratories