Application of the Fluorescent Antibody Technique to Dried and Eluted Blood: A Comparison with FTA, TPI and Lipoidic Antigen Tests of the Tests of the Serum

Abstract

This report describes the preliminary results obtained with FTA using discs of Canson (no. 435) blotting paper. After absorption, drying and storage, 150 eluted samples taken from both normal and infected subjects were tested by FTA. The reactivity to FTA, TPI was studied in parallel to lipoid tests of sera obtained from the same subjects by venous puncture. The variations of sensitivity, of specificity and of reproducibility noted in the discs used in the FTA100 technique were insignificant; the results were practically identical to those of FTA100 used on sera obtained by venous puncture. No deterioration of the antibody was observed as a result of storage of discs at 20-25 deg C for periods ranging from several days up to 60 days.

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Document Details

Document Type
Technical Report
Publication Date
Feb 15, 1968
Accession Number
AD0842906

Entities

People

  • A. Hamelin
  • A. Vaisman
  • L. Descombes
  • T. Guthe

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Biological Laboratories
  • Blood
  • Buffers (Chemistry)
  • Filter Paper
  • Government (Foreign)
  • Governments
  • Health
  • Health Services
  • Infection
  • Paper
  • Public Health
  • Reactivities
  • Reproducibility
  • Sensitivity
  • Syphilis
  • United States

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