Demonstration by Acrylic Gel Electrophoresis of the Specificity of Luxol Blue in the Reaction with Phospholipid (Demonstration Par L'Electrophores En Gel D'Acryle De La Specificite Du Bleu Luxol En Tant Que Reactif Des Phospholipides)

Abstract

We have confirmed the preferential fixation of Luxol blue to lipoproteins rich in phospholipids, such as alpha-lipoproteins and thromboplastin, and the complete absence of staining with other native or lipid- free proteins. The mode of fixation does not occur at the level of the active groups responsible for electrophoretic mobility of lipoproteins, as shown by the simultaneous electrophoresis of serum prestained with Sudan blue, Nile blue, and Luxol fast blue.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1968
Accession Number
AD0843447

Entities

People

  • R. Delcourt

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Acetic Acid
  • Aqueous Solutions
  • Blood Proteins
  • Electrophoresis
  • Fluids
  • Gel Electrophoresis
  • Government (Foreign)
  • Governments
  • Hydroxides
  • Lipids
  • Lipoproteins
  • Maryland
  • Membrane Lipids
  • Proteins
  • Sodium Hydroxide
  • United States

Fields of Study

  • Biology
  • Chemistry

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Molecular and Cellular Biochemistry
  • Small Business Innovation Research Program (SBIR) EDI Research and Innovation.