The Serodiagnosis of Syphilis by Immunofluorescence (In Regard to 207 Examined Sera)
Abstract
The serum of a syphilitic subject contains antitreponema antibodies which are globulins. If one fixes a treponeme (for example treponemes of Nichol's strain, identical to those used in Nelson's test and obtained by passages through rabbit testicles) suspension on a slide, these globulin-antibodies will fix themselves on the spirochete, but also act as antigen for an antiglobulin-human serum which also fixes itself on the treponeme antigen-antibody complex. Since the antiglobulin-human serum is combined with a fluorescein derivative, the treponeme becomes fluorescent under ultraviolet examination. On the other hand, in the absence of antibody, that is to say in the study of a nonsyphilitic serum, the antiglobulin is not fixed and the antigen remains nonfluorescent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 04, 1968
- Accession Number
- AD0843448
Entities
People
- C. Mengus
- G. Richer
- J. Larribaud
- J. Migne
- J. Teillard
Organizations
- United States Army Biological Warfare Laboratories