Immunofluorescent Cell-Counting Assay of Rift Valley Fever Virus
Abstract
Rift Valley fever virus was quantitatively assayed by enumeration of cells containing fluorescent viral antigens after infection of L cell monolayers. The efficiency and speed of virus attachment were markedly enhanced when augmented by centrifugal force. Approximately 97% of virus inoculum attached to cells within 10 minutes. Thus, a proportionality was obtained between the number of infected cells and volume of inoculum. Virus penetration into cells was linear and complete within 30 minutes at 35 deg C. From observations on the sequential development of viral antigen within cells and fluorescent cell counts, infected cells were enumerated as early as 12 hours after inoculation of cell monolayers. A linear function was demonstrated between infected cell counts and virus concentration. The immunofluorescent cell-counting assay of Rift Valley fever virus was more precise, sensitive, and rapid than assays based on intraperitoneal inoculation of mice or cytopathic effects in cell cultures.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1968
- Accession Number
- AD0843595
Entities
People
- Nicholas Hahon