The Development of Mutations of Tobacco Mosaic Virus by the Chemical Treatment of its Nucleic Acid in Vitro
Abstract
In order to demonstrate the genesis of mutants in vitro, conditions were chosen under which a strong increase occurs in the number of necrotic lesions caused by the mutants on Java tobacco. Owing to this increase, the number of mutants multiplies 20 times over the original value in an area where the infectivity has decreased by only one half. This precludes selective processes. Nor may the rise be attributed to a higher degree of probability of the mutants' assertiveness due to dilution or inactivation, as frequently noted (Mundry 1957 a). Therefore the mutants were induced by treatment with nitrite.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0843942
Entities
People
- A. Gierer
- K. W. Mundry
Organizations
- United States Army Biological Warfare Laboratories