Studies with Fluorescence-Labeled Antibodies. V. Simultaneous Demonstration of Several Antigens by Means of Variously Colored Fluorescent Antibodies, Using Proteus Morganii and Bacillus Cereus as Examples

Abstract

The labeling of immune gamma globulins with the sulfochlorides of various fluorescent dyes is described. Sulfo-rhodamine B, a pigment with reddish-yellow fluorescence, proved to be a feasible contrasting agent for hydroxy-pyrene-trisulfonic acid chloride and other green-fluorescent labeling substances. P. morganii and Bc. cereus were used to demonstrate that simultaneous specific staining with a mixture of variously labeled antibodies is possible. The potential of the method and its advantages and disadvantages, are discussed.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1968
Accession Number
AD0843966

Entities

People

  • G. Poetschke
  • H. Uehleke
  • L. Killisch

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Counter WMD

DTIC Thesaurus Topics

  • Acids
  • Antibodies
  • Antigens
  • Biological Staining And Labeling
  • Biomedical And Dental Materials
  • Buffers (Chemistry)
  • Dyes
  • Fluorescence
  • Fluorescent Dyes
  • Gamma Globulin
  • Globulins
  • Government (Foreign)
  • High Pressure
  • Immune Serums
  • Immunoglobulins
  • Laser Dyes
  • Proteins

Fields of Study

  • Biology

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Microbial Pathology
  • Systems Analysis and Design