A Micro-Method of Protein Analysis
Abstract
The determination of protein by means of Weichselbaum's (1) biuret method is too inexact when dealing with small quantities of protein (less than 200 microgram/ml initial reactant), owing to the low sensitivity of the color reaction. Although we have used this method for protein analysis of enzymatic preparations from amoebae in a simple micro-modification (2) (initial reactant 200 micron 1 with at least 11 microgram protein), we nevertheless have searched for a more sensitive colorimetric method. Nielsen (3) recently reported on a method in which the Cu bound by protein in the biuret reaction is analyzed colorimetrically with sodium-diethyl-dithiocarbamate (DDTC). The method becomes about 50 times more sensitive thereby. This paper describes a modification of this method which permits protein analysis of very small volumes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1968
- Accession Number
- AD0845740
Entities
People
- H. Mattenheimer
Organizations
- United States Army Biological Warfare Laboratories