Estimation of Precipitating Antibody Using a Turbidimetric Technique
Abstract
The development of turbidity resulting from the precipitation between bovine serum albumin (BSA) and anti-BSA serum was studied in the spectrophotometer. The equivalence ratio and the titer of precipitating antibody could be determined by this method. Data obtained with the turbidimetric method correlated well with those obtained using the classical tube precipitin determination. Turbidimetric measurements were also applied successfully to the measurement of antibody titer in two other systems. Antibody titer can be determined with this method in about an hour as compared with the 3 or more days required by the tube test. In addition, this technique requires substantially less reagent than other methods. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1968
- Accession Number
- AD0848652
Entities
People
- Earl W. Harris
- Sidney Yaverbaum
- William F. Vincent