Mode of Action of 5-Azacytidine on Arboviruses
Abstract
Using eastern equine encephalitis (EEE) virus or Ets-4 (a temperature-sensitive mutant of EEE) in pulse-labeled experiments, 5-AzaC did not show any significant effect on the initiation or rate of total RNA synthesis but did lower the cumulative uptake of C14-uridine in 6-hour infected cells. The lower cumulative uptake may be the result of breakdown of RNA synthesized in the presence of 5-AzaC as hypothesized for bacteria. Sucrose gradient analysis of the different viral RNA species in infected cells revealed a somewhat diminished 20S and 27S RNA fraction in 6-hour infected cultures treated with 25 microgram/ ml 5-AzaC but the 45S fraction was not affected. The viral RNA synthesized in the presence of 5-AzaC was significantly labile upon phenol extraction, having only 2 to 5% of the infectivity of controls, yet it showed approximately the same quantity of 45S RNA; the latter is normally the most infectious of the known species of viral RNA. The data accumulated on the mutagenic and inhibitory effects of 5-AzaC suggest that it acts directly on viral RNA and support the notion yet to be proved that it is incorporated into the viral RNA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1969
- Accession Number
- AD0848890
Entities
People
- Arthur Brown
- Sidney Halle