Nonviable Vee Hemagglutinin Prepared from Tissue Cultures by Gamma Radiation

Abstract

Hemagglutinins (HA) of Venezuelan equine encephalomyelitis (VEE) virus were produced in Maitland-type cultures of chicken embryo (MTCE) and in monolayers of hamster kidney, McCoy, and human diploid strain W1-38 cells. Optimal pH values for the demonstration of Ha in MTCE preparations ranged from 5.65 to 5.85. Exposure of MTCE HA to 8 x 1,000,000 r of gamma rays destroyed the infectivity of the antigen while most of the HA activity was retained. Irradiated HA performed satisfactorily in hemagglutination-inhibition tests of human and animal sera.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1969
Accession Number
AD0852034

Entities

People

  • Morton Reitman

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Arboviruses
  • Cells
  • Encephalitis
  • Equine Encephalitis
  • Gamma Rays
  • Influenza
  • Ionizing Radiation
  • Laboratory Animals
  • Monomolecular Films
  • Radiation
  • Tissue Culture
  • Venezuelan Equine Encephalomyelitis
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Nuclear and Radiation Engineering.
  • Virology (or Medical Virology).