Preparation of Noninfectious Arbovirus Antigens

Abstract

Noninfectious arbovirus antigens were extracted from saline suspensions of infected suckling mouse brain that were buffered with tris(hydroxymethyl)aminomethane and treated with beta-propiolactone (BPL). The activity and stability of several antigens were enhanced by altering the buffering system and by rapid, continuous passage of the virus in suckling mice. For several viruses of groups A and B, the titers of antigens extracted with borate saline-BPL (BS-BPL) were comparable to titers of antigens extracted with sucrose-acetone-BPL. Results from some ungrouped and Bunyamwera arboviruses required the development of unique and more sophisticated procedures for the preparation of suitable antigens.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1969
Accession Number
AD0852676

Entities

People

  • Orville M. Brand
  • William P. Allen

Tags

Communities of Interest

  • Counter IED

DTIC Thesaurus Topics

  • Antigens
  • Arboviruses
  • Biochemistry
  • Biological Sciences
  • Chemical Compounds
  • Chemistry
  • Extraction
  • Hemagglutination Tests
  • Laboratory Animals
  • Lactones
  • Literature
  • Materials
  • Propiolactones
  • Rift Valley Fever
  • Rift Valleys
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Virology (or Medical Virology).