Chromosome Mapping of Pasteurella pseudotuberculosis by Interrupted Membrane Mating

Abstract

Pasteurella pseudotuberculosis F'lac, after growth in brain heart infusion to obtain maximum F piliation, mated on membranes with three different multiple auxotrophs of P. pseudotuberculosis F(-). Samples of mating pairs were periodically separated by vigorous mixing and plated on selective media containing nalidixic acid to counderselect against the donor strain. The transfer of six markers was observed within 65 minutes after mating, resulting in a partial chromosome map. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1969
Accession Number
AD0857267

Entities

People

  • Harold B. Stull
  • William D. Lawton

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Anatomy
  • Anti-Bacterial Agents
  • Bacteria
  • Biological Sciences
  • Biology
  • Cells
  • Escherichia
  • Escherichia Coli
  • Gammaproteobacteria
  • Genes
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Infusions
  • Maryland
  • Microorganisms

Fields of Study

  • Biology

Readers

  • Immunology
  • Mathematics or Statistics
  • Molecular Genetics