Interaction of Venezuelan Equine Encephalomyelitis Virus with Neutralizing Antibody: 3. The Serum Neutralization Anti-IgG Test

Abstract

A precise, reproducible, and sensitive serum neutralization test was developed to estimate Venezuelan equine encephalomyelitis (VEE) serum- neutralizing antibodies within 24 hours. The test is dependent on both the interactions of virus with antiviral serum and the resultant complex with anti- gamma globulin (IgG) antibodies. The 50% serum-neutralizing end point was calculated from the reduction of number of fluorescent cells in McCoy cell monolayers resulting from the neutralization of infective viral particles by antibodies. In comparative estimates of neutralizing antibodies of human and monkey sera, the sensitivity of the serum neutralization anti-IgG test was several hundred times greater than that of the conventional mouse serum neutralization test.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1969
Accession Number
AD0858310

Entities

People

  • Nicholas Hahon

Tags

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Antigens
  • Arbovirus Infections
  • Biomedical And Dental Materials
  • Cells
  • Culture Techniques
  • Gamma Globulin
  • Globulins
  • Immune Serums
  • Incubation
  • Medical Personnel
  • Polymeric Films
  • Rhesus Monkeys
  • Venezuelan Equine Encephalomyelitis
  • Virion
  • Viruses

Fields of Study

  • Biology

Readers

  • Regression Analysis.
  • Virology (or Medical Virology).