The Requirement for Coenzyme A in the Phosphoroclastic Reaction of Anaerobic Bacteria.
Abstract
A variety of bacteria which degrade pyruvate by the 'phosphoroclastic reaction' were examined with respect to the role of CoA in this reaction. This strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levals of CoA for the Co2-pyruvate exchange and acetoin forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulpho-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1969
- Accession Number
- AD0858934
Entities
People
- R. B. Hespell
- R. P. Moftlock
- Robiya Joseph
Organizations
- University of Massachusetts Amherst