The Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38
Abstract
We demonstrated that Venezuelan equine encephalomyelitis (VEE) replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of as high as 10 to the 9.9th power MICLD50/ml were obtained after a 48- hour incubation period at low passage levels in Eagle basal medium supplemented with 3% calf serum. VEE virus replicated poorly in serum-free medium. Propagation of the virus was accompanied by the production of hemagglutinin and cytopathogenic effect. These studies suggest that the WI-38 strain might be an appropriate source for the production of VEE virus for vaccines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1969
- Accession Number
- AD0860417
Entities
People
- Leonard Green
- Morton Reitman