An Improved Chemically Defined Culture Medium for Strain L Mouse Cells Based on Growth Responses to Graded Levels of Nutrients
Abstract
Nutritional factors were evaluated for effects on growth of mouse fibroblast cells in suspension in a chemically defined medium. Quantitative requirements for each essential amino acid, choline, inorganic phosphate, iron, and zinc were established. An improved chemically defined medium was formulated on the basis of the findings yielding populations of L cells in excess of 5,000, 000/ml without nutrient replenishment. When spent medium was replaced periodically, yields approaching 30 x 10 to the sixth power cells/ml were attained. The efficiency of utilization of most amino acids in the new medium appears to be twofold to threefold better than results reported by others.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1969
- Accession Number
- AD0860422
Entities
People
- Kiyoshi Higuchi