Studies on Microbial Degradation of Toxic Phosphorus Esters. Part 1. Detoxification of O,O-Dimethyl-O-(2-carbomethoxy-1-methylvinyl) Phosphate by Soil Bacteria.

Abstract

In an attempt to find a new method for detoxifying cholinesterase-inhibiting, organophosphorus compounds, studies were undertaken to isolate bacteria capable of degrading O,O-dimethyl-O-(2-carbomethoxy-1-methylvinyl) phosphate (Phosdrin). Degradation of the insecticide was determined by gas chromatography, while decreased toxicity was confirmed by measuring cholinesterase activity with a recording pH stat. Of the thirty cultures screened, two showed 80-90% degradation of the original 20 ppm Phosdrin in 10 days, with a corresponding decrease in toxicity. One of the cultures was isolated from soil collected on Eglin Air Force Base, Florida, and the other as a laboratory contaminant. The bacteria were identified as two different strains of Bacillus megaterium. (Author)

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1969
Accession Number
AD0860651

Entities

People

  • B. C. Wolverton
  • Carolyn Madura

Tags

DTIC Thesaurus Topics

  • Air Force
  • Air Force Facilities
  • Bacteria
  • Chemical Compounds
  • Chromatography
  • Degradation
  • Detoxification
  • Environmental Pollutants
  • Gas Chromatography
  • Insecticides
  • Organic Compounds
  • Organophosphorus Compounds
  • Phosphorus
  • Toxicity

Fields of Study

  • Agricultural and Food sciences

Readers

  • Analytical Chemistry
  • Groundwater Contamination Remediation.
  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation