Some Characteristics of Riboflavin Chemiluminescence
Abstract
Data are presented that describe some characteristics of riboflavin chemiluminescence in the dark in the presence of hydrogen peroxide and osmium trichloride. The reaction, in terms of the light intensity produced, is affected by type of buffer, pH, and concentration of reactants. Light intensity is directly proportional to the concentration of riboflavin, and a 1,000-fold increase in relative light intensity is produced when osmium is present. The optimum pH for the reaction in phosphate buffer is 8.0 and in sodium hydroxide, 12. Spectral studies of the riboflavin chemiluminescent reaction show that the emitting species has a maximum intensity of emission at a wavelength different from the fluorescence maximum of riboflavin. The observed 495-millimicron maximum is similar to the maximum observed for the luminescent bacterium, Achromobacter fischeri.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1969
- Accession Number
- AD0863653
Entities
People
- Harold A. Neufeld
- Philip B. Shevlin
- Richard D. Towner