Further Studies on Control of RNA Synthesis by a Mutant of Eastern Equine Encephalitis Virus
Abstract
A temperature-sensitive mutant derived from eastern equine encephalitis (EEE) virus was found to induce the synthesis of a large amount of viral RNA in infected chick embryo (CE) cells. The mutant, designated Ets-4, synthesized two to four times more RNA than did the parent; however, its yield of mature virus was 0.5 to 1.0 log10 units less than that of EEE VIRUS) simultaneous infection of CE cells with parent and mutant viruses reduced the amount of RNA synthesized by Ets-4 to a level that approximated that of EEE alone. The viral RNA polymerase activity induced in CE cells infected with Ets-4 virus was higher than with the parent virus. The presence of EEE virus in Ets-4 - infected cells, however, reduced the amount of RNA polymerase synthesized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1969
- Accession Number
- AD0864552
Entities
People
- Arthur Brown
- Eugene Zebovitz